
CUSTODIOL
Decades of Trust
Leading the way in myocardial protection and organ preservation
History of CUSTODIOL
Dr. Franz Köhler, in collaboration with Prof. Bretschneider, developed the initial formulation of CUSTODIOL.
Dr. Franz Josef Köhler
1909 - 1984
Prof. Hans-Jürgen Bretschneider
1922 - 1993

CUSTODIOL HTK Solution
Histidine-Tryptophan-Ketoglutarate Solution
Indicated for:
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(MICS) Minimally Invasive Cardiac Surgery
Coronary Artery Bypass (CABG)
Paediatric Heart Surgery
Heart Transplantation
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Standard preservation solution for abdominal organs in the Eurotransplant region
Preferred by leading transplant centres in North and South America, and increasingly in Asia and Africa
Suitable for multi-organ procurement (heart, liver, kidney, pancreas, composite tissue)
Reliable protection for organs, including those from non-heart beating donors
"Ready-to-use"
Storage condition: 2 -8°C
Proven Track Record of CUSTODIOL
Global Approval
Registered and approved for use in many countries worldwide.
Extensive Clinical Evidence
Over 1,000 scientific publications
Widespread Use
Used in over 3 million procedures worldwide.
Why Choose CUSTODIOL?
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Single-Shot Protection for up to 180 minutes. (1)
Superior Myocardial Protection. (2-10)
Reduced Hospital Stay and Faster Recovery (2, 11-13)
- Less intraoperative time compared to traditional repetitive cardioplegia. (14, 15)
- Cost-effective when compared with blood cardioplegia. (15)
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Facilitates rapid perfusion and cooling of organs due to low viscosity. (6,16)
Sustains energy supply (7, 17)
Exhibits strong antioxidant effects (18,19)
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Safe, Simple and Ready to use (20)
Standardized formulation (14)
Manufactured to GMP.
For more information, visit:
Holistic support and dedicated training for our trusted partners.
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Preusse, C.J., Time to Reconsider the ‘Cardioplegia Paradigm’. Perfusionist, 2018. 42: p. 1-4.
Albadrani, M., Histidine-tryptophan-ketoglutarate solution versus multidose cardioplegia for myocardial protection in cardiac surgeries: a systematic review and meta-analysis. J Cardiothorac Surg, 2022. 17(1): p. 133.
Preusse, C.J., CUSTODIOL® Cardioplegia: A Single-Dose Hyperpolarizing Solution. J Extra Corpor Technol, 2016. 48: p. 15-20.
Lück, S., lntraoperative Myokardprotektion mittels Bretschneider-Kardioplegie. Kardiotechnik, 2016. 04: p. 1-4.
Doetsch, N., Habilitation – Systemische Auswirkungen Histidingepufferter Kardioplegischer Lösung HTK nach Bretschneider. 1993: Pabst Verlag.
Gubernatis, G., et al., HTK-solution (Bretschneider) for human liver transplantation. First clinical experiences. Langenbecks Arch Chir, 1990. 375(2): p. 66-70.
Garfinkel, L., R.A. Altschuld, and D. Garfinkel, Magnesium in cardiac energy metabolism. J Mol Cell Cardiol, 1986. 18(10): p. 1003-13.
Braathen, B., et al., One single dose of histidine-tryptophan-ketoglutarate solution gives equally good myocardial protection in elective mitral valve surgery as repetitive cold blood cardioplegia: a prospective randomized study. J Thorac Cardiovasc Surg, 2011. 141(4): p. 995-1001.
Arslan, A., et al., Low-dose histidine-tryptophan-ketoglutarate solution for myocardial protection. Transplant Proc, 2005. 37(7): p. 3219-22.
Kammerer, I., et al., Myocardial Protection in Minimally Invasive Mitral Valve Surgery: Comparison of the Cold-Blood Cardioplegia of the Bretschneider Solution and the Warm-Blood Cardioplegia of the Calafiore Protocol. Archives of Clinical and Experimental Surgery (ACES), 2012. 1(1).
Ali, I., et al., Efficacy of Histidine-Tryptophan-Ketoglutarate Solution Versus Blood Cardioplegia in Cardiac Surgical Procedures: A Randomized Controlled | Parallel Group Study. Heart Surg Forum, 2021. 24(1): p. E170-e176.
Mercan, I., et al., Comparison between the Effects of Bretschneider's HTK Solution and Cold Blood Cardioplegia on Systemic Endothelial Functions in Patients who Undergo Coronary Artery Bypass Surgery: a Prospective Randomized and Controlled Trial. Braz J Cardiovasc Surg, 2020. 35(5): p. 634-643.
Vivacqua, A., et al., Single-dose cardioplegia protects myocardium as well as traditional repetitive dosing: A noninferiority randomized study. J Thorac Cardiovasc Surg, 2020. 159(5): p. 1857-1863 e1.
Giordano, R., et al., CUSTODIOL® Solution and Cold Blood Cardioplegia in Arterial Switch Operation: Retrospective Analysis in a Single Center. Thorac Cardiovasc Surg, 2016. 64(1): p. 53-8.
Hummel, B.W., et al., Myocardial Protection and Financial Considerations of CUSTODIOL® Cardioplegia in Minimally Invasive and Open Valve Surgery. Innovations (Phila), 2016. 11(6): p. 420-424.
Wilson, C.H., et al., Comparison of HTK and hypertonic citrate to intraarterial cooling in human non-heart-beating kidney donors. Transplant Proc, 2007. 39(2): p. 351-2.
Tangvoraphonkchai, K. and A. Davenport, Magnesium and Cardiovascular Disease. Adv Chronic Kidney Dis, 2018. 25(3): p. 251-260.
Powell, W.J., Jr., et al., The protective effect of hyperosmotic mannitol in myocardial ischemia and necrosis. Circulation, 1976. 54(4): p. 603-15.
Waskowski, J., et al., Mannitol for the Prevention of Peri-Operative Acute Kidney Injury: A Systematic Review. Eur J Vasc Endovasc Surg, 2019. 58(1): p. 130-140.
Holper, K., et al., Technical refinements in myocardial protection: infants--adults. Thorac Cardiovasc Surg, 1998. 46 Suppl 2: p. 292-5; discussion 296-7.
